Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Phosphodiester Linkages01:01

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid Aminoferrocenes With Nitrate Esters for In Situ Peroxynitrite Generation: Complex Interplay Between Redox Chemistry, NO-Donor Reactivity, and Aminoferrocene Activation Influences In Vitro Anticancer Activity.

Chembiochem : a European journal of chemical biology·2026
Same author

Versatile SI-ATRP Growth of Methacrylate Brushes on Superparamagnetic Iron Oxide Nanoparticles Enables Methotrexate-Mediated Antineoplastic Activity in MCF-7 Cells.

Pharmaceutics·2026
Same author

Dextran-driven hyperbranched black gold nanoparticles templated by guanosine quartets as broadband absorbers for photothermal therapy.

International journal of biological macromolecules·2026
Same author

Polyethyleneimine-Directed In Situ Gold Deposition on Gallium Nitride Nanoparticles for Enhanced Electrochemical Detection of Erythromycin.

International journal of molecular sciences·2026
Same author

Development of the European Veterinary Medicines Gaps and Needs Compass for Sheep and Goats Based on Online Survey and Expert Knowledge Elicitation.

Veterinary sciences·2026
Same author

A "turn-on" chemodosimeter for detection of Cu<sup>2+</sup> in living cells.

Dalton transactions (Cambridge, England : 2003)·2025

Related Experiment Video

Updated: Jul 3, 2026

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

Red light-activated phosphorothioate oligodeoxyribonucleotides.

Alexandru Rotaru1, János Kovács, Andriy Mokhir

  • 1Inorganic Chemistry Institute, University of Heidelberg, In Neuenheimer Feld 270, 69120 Heidelberg, Germany.

Bioorganic & Medicinal Chemistry Letters
|July 16, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed novel

More Related Videos

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Related Experiment Videos

Last Updated: Jul 3, 2026

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Area of Science:

  • Oligonucleotide chemistry
  • Photochemistry
  • Molecular biology

Background:

  • Phosphorothioate oligodeoxyribonucleotides are widely used in research and therapeutics.
  • Controlling the binding activity of these molecules is crucial for targeted applications.
  • Existing methods for controlling binding are often limited or lack spatiotemporal precision.

Purpose of the Study:

  • To create light-activatable phosphorothioate oligodeoxyribonucleotides.
  • To develop a system for triggering nucleic acid binding with red light.
  • To demonstrate a novel method for 'caging' and uncaging oligonucleotide function.

Main Methods:

  • Synthesis of hairpin-structured phosphorothioate oligodeoxyribonucleotides with a singlet oxygen-sensitive linker.
  • Photochemical activation using red light and chlorine e6 (photosensitizer).
  • Assessment of nucleic acid binding ability before and after light irradiation.

Main Results:

  • The synthesized compounds remained inactive in the dark, showing no binding to complementary nucleic acids.
  • Upon red light irradiation in the presence of chlorine e6, the linker was cleaved.
  • Cleavage resulted in the formation of single-stranded oligodeoxyribonucleotides with potent binding capabilities.
  • This represents the first demonstration of 'caged' phosphorothioate oligodeoxyribonucleotides activated by red light.

Conclusions:

  • Light-triggered release of functional phosphorothioate oligodeoxyribonucleotides is achievable.
  • This technology offers precise spatiotemporal control over oligonucleotide binding.
  • The developed 'caged' oligonucleotides hold potential for advanced molecular biology tools and therapeutics.