Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 3, 2026

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

HTRF-based assay for microsomal prostaglandin E2 synthase-1 activity.

Eric R Goedken1, Andrew I Gagnon, Gary T Overmeyer

  • 1Abbott Bioresearch Center, Worcester, Massachusetts 01605, USA. eric.goedken@abbott.com

Journal of Biomolecular Screening
|July 16, 2008
PubMed
Summary

A new assay simplifies measuring microsomal prostaglandin E2 synthase-1 (mPGES-1) activity, crucial for developing anti-inflammatory drugs targeting prostaglandin E2 (PGE2) production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Induction of human pruriceptors from pluripotent stem cells via transcription factors.

Stem cell reports·2026
Same author

An implantable mechano-electro cascade platform synchronizes neuro-muscle repair.

Nature communications·2026
Same author

Aryl hydrocarbon receptor: a multifaceted environmental sensor in liver homeostasis and disease.

Frontiers in medicine·2026
Same author

HR-Mamba: Building Footprint Segmentation with Geometry-Driven Boundary Regularization.

Sensors (Basel, Switzerland)·2026
Same author

Infrared land surface emissivity dynamics in the Taklimakan desert from 2001 to 2023.

Scientific reports·2026
Same author

Resolving the mRNA Encapsulation-Release Trade-off via Compensatory Forces in Engineered Ionizable Lipids.

Advanced materials (Deerfield Beach, Fla.)·2025

Area of Science:

  • Biochemistry
  • Enzymology
  • Pharmacology

Background:

  • Microsomal prostaglandin E2 synthase-1 (mPGES-1) produces prostaglandin E2 (PGE2), a key mediator in inflammatory responses.
  • mPGES-1 is upregulated during inflammation, making it a potential therapeutic target for antiarthritic treatments.
  • Existing in vitro assays for mPGES-1 are challenging due to the instability of the prostaglandin H2 (PGH2) substrate.

Purpose of the Study:

  • To develop a robust and convenient assay for measuring mPGES-1 enzyme activity.
  • To enable characterization of enzyme preparations and assessment of inhibitor potency.
  • To facilitate the discovery of novel anti-inflammatory compounds targeting mPGES-1.

Main Methods:

  • A plate-based competition assay utilizing homogenous time-resolved fluorescence (HTRF).

More Related Videos

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Colorimetric Assessment of Deiodinase 1 Activity in Human Liver Microsomes Using the Sandell-Kolthoff Reaction
08:00

Colorimetric Assessment of Deiodinase 1 Activity in Human Liver Microsomes Using the Sandell-Kolthoff Reaction

Published on: April 10, 2026

Related Experiment Videos

Last Updated: Jul 3, 2026

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Colorimetric Assessment of Deiodinase 1 Activity in Human Liver Microsomes Using the Sandell-Kolthoff Reaction
08:00

Colorimetric Assessment of Deiodinase 1 Activity in Human Liver Microsomes Using the Sandell-Kolthoff Reaction

Published on: April 10, 2026

  • The assay measures the production of PGE2 catalyzed by mPGES-1.
  • It is designed for simplicity, requiring minimal washes and tolerating DMSO concentrations up to 10%.

    • Main Results:

    • The developed assay accurately measures mPGES-1 activity.
    • It is suitable for screening potential mPGES-1 inhibitor compounds.
    • The assay's convenience and robustness facilitate enzyme characterization.

    Conclusions:

    • A novel, user-friendly HTRF-based assay for mPGES-1 activity has been established.
    • This assay is effective for evaluating mPGES-1 inhibitors and characterizing enzyme preparations.
    • The assay supports the development of targeted anti-inflammatory therapies.