Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insights into the complexity of SARS-CoV-2 M<sup>pro</sup> inhibition: Ebselen and its derivatives impair dimerisation of the enzyme.

Journal of enzyme inhibition and medicinal chemistry·2026
Same author

Exploiting ALDH1A2 and ALDH1A3 isoform variability for crystallisation screening.

Biochemical and biophysical research communications·2025
Same author

Allostery in homodimeric SARS-CoV-2 main protease.

Communications biology·2024
Same author

One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse.

Nature communications·2024
Same author

Antitarget, Anti-SARS-CoV-2 Leads, Drugs, and the Drug Discovery-Genetics Alliance Perspective.

Journal of medicinal chemistry·2023
Same author

Analysis of the phosphoproteome of CK2<i>α</i><sup>(-/-)</sup>/Δ<i>α'</i> C2C12 myoblasts compared to the wild-type cells.

Open biology·2023
Same journal

High glucose-induced mitochondrial fission promotes Müller cell activation via suppression of the Hippo pathway.

Molecular and cellular biochemistry·2026
Same journal

Correction to: Estradiol inhibits vascular endothelial cells pro-inflammatory activation induced by C-reactive protein.

Molecular and cellular biochemistry·2026
Same journal

Galectin-3, transforming growth factor beta 1, and brain natriuretic peptide in cardiac remodeling under hyperlipidemic and hyperglycemic stress.

Molecular and cellular biochemistry·2026
Same journal

Ellagic acid from the traditional Chinese medicinal herb Scutellaria barbata may accelerates apoptosis in hepatic stellate cells during liver fibrosis via inhibiting miR-182-5p.

Molecular and cellular biochemistry·2026
Same journal

GeneQuantify: a web-based tool for qPCR gene expression and copy number variation analysis.

Molecular and cellular biochemistry·2026
Same journal

NCAPG reprograms glycolytic and lipid metabolism by sustaining glycerophospholipid flux in small-cell lung cancer.

Molecular and cellular biochemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2026

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
11:11

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

A structural insight into CK2 inhibition.

Marco Mazzorana1, Lorenzo A Pinna, Roberto Battistutta

  • 1Department of Biological Chemistry, University of Padua, Padua, Italy.

Molecular and Cellular Biochemistry
|July 16, 2008
PubMed
Summary
This summary is machine-generated.

Protein kinase CK2, a target for cancer therapy, has unique features making it drug-resistant. New inhibitors targeting its ATP-binding site show promise by exploiting hydrophobic interactions and conserved water molecules for selective binding.

More Related Videos

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/&#945;
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

Related Experiment Videos

Last Updated: Jul 3, 2026

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
11:11

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/&#945;
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • The Ser/Thr protein kinase CK2 is a pleiotropic enzyme with constitutive activity.
  • CK2 plays crucial roles in normal cellular processes, including cell survival.
  • CK2 also contributes to tumor progression, making it a significant therapeutic target.

Purpose of the Study:

  • To review recent studies on inhibitors targeting the CK2 ATP-binding site.
  • To highlight the unique features of CK2, such as its ability to use both ATP and GTP and resistance to staurosporine.
  • To discuss the binding characteristics and selectivity of various chemical inhibitor families.

Main Methods:

  • Analysis of CK2 crystal structures.
  • Investigation of inhibitor binding to the CK2 ATP-binding site.
  • Evaluation of inhibitor potency and selectivity.

Main Results:

  • Selective inhibitors with nanomolar to low micromolar potency were identified.
  • Hydrophobic interactions with the CK2 binding cleft are a key binding contributor.
  • Conserved water molecules near Lys68 and bulky residues (Ile66, Ile174) influence ligand orientation and selectivity.

Conclusions:

  • CK2 inhibitors can be developed by targeting the ATP-binding site.
  • Understanding the structural features of the active site is critical for designing selective inhibitors.
  • These findings provide a basis for developing novel CK2-targeted therapeutics.