Injectable iron-modified apatitic bone cement intended for kyphoplasty: cytocompatibility study

Area of Science:

• Biomaterials Science
• Cell Biology
• Orthopedic Surgery

Background:

• Osteoporotic vertebral compression fractures require effective treatments.
• Injectable bone cements offer minimally invasive solutions.
• Calcium phosphate cements are widely used biomaterials.

Purpose of the Study:

• To evaluate the cytocompatibility of novel injectable iron-modified calcium phosphate cements (IM-CPCs).
• To assess the impact of IM-CPCs on human epithelial (HEp-2) cell adhesion, proliferation, and morphology.
• To determine the suitability of IM-CPCs for kyphoplasty applications.

Main Methods:

• Human epithelial (HEp-2) cells were cultured on IM-CPCs.
• Quantitative MTT assay was used to assess cell viability and proliferation.
• Scanning electron microscopy (SEM) was employed to examine cell morphology and adhesion.

Main Results:

• Cell adhesion and viability were not adversely affected by IM-CPCs over time or by varying iron concentrations.
• SEM analysis revealed that HEp-2 cells successfully adhered, spread, and exhibited normal morphology on the IM-CPCs.
• No dose-dependent negative effects of iron concentration on cell behavior were observed.

Conclusions:

• The novel injectable IM-CPCs exhibit favorable cytocompatibility.
• IM-CPCs support healthy cell adhesion, proliferation, and morphology.
• These findings support the potential use of IM-CPCs in kyphoplasty for treating osteoporotic vertebral compression fractures.