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Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

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Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes

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Extractive lactic acid fermentation in poly (ethyleneimine)-based aqueous two-phase system.

Y J Kwon¹, R Kaul, B Mattiasson

¹Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, S-221 00 Lund, Sweden.

Biotechnology and Bioengineering

|May 5, 1996

Summary

Aqueous two-phase systems using poly(ethyleneimine) (PEI) and (hydroxyethyl) cellulose (HEC) enhance lactic acid fermentation. This method significantly increases lactate and biomass production compared to conventional methods.

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Area of Science:

Biotechnology
Biochemical Engineering

Background:

Lactic acid fermentation is crucial for various industries.
Improving yield and productivity in lactic acid fermentation remains a challenge.
Aqueous two-phase systems (ATPS) offer potential for bioprocess intensification.

Purpose of the Study:

To evaluate the efficacy of a poly(ethyleneimine) (PEI) and (hydroxyethyl) cellulose (HEC) based ATPS for extractive lactic acid fermentation.
To compare the performance of ATPS with conventional monophasic systems for lactic acid production using Lactococcus lactis.

Main Methods:

Batch and continuous fermentation experiments were conducted using Lactococcus lactis.
The study employed a two-phase system composed of PEI (polycation) and HEC (uncharged polymer).
Fermentations were performed with varying glucose concentrations and phosphate buffer conditions, with and without external pH control.

Main Results:

Without pH control, ATPS resulted in 3-4 times higher lactate and biomass production compared to monophasic medium.
Lactic acid preferentially partitioned to the PEI-rich phase, while cells shifted to this phase post-fermentation.
With pH control, ATPS yielded 1.3 times higher cell density, especially at high glucose concentrations (50-100 g/L).
Continuous fermentation in ATPS with higher phosphate concentration improved cell density, product yield, and productivity.

Conclusions:

PEI/HEC based ATPS significantly enhances lactic acid fermentation performance, particularly in terms of yield and biomass.
Cell partitioning behavior in the two-phase system is influenced by pH and buffer conditions.
ATPS demonstrates considerable potential for improving the efficiency of industrial lactic acid production.