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Updated: Jul 3, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

G-quadruplex DNA assemblies: loop length, cation identity, and multimer formation.

Nicolas Smargiasso1, Frédéric Rosu, Wei Hsia

  • 1Mass Spectrometry Laboratory, GIGA-Research, University of Liège, Belgium.

Journal of the American Chemical Society
|July 17, 2008
PubMed
Summary
This summary is machine-generated.

Loop length and cation type significantly influence G-quadruplex structure and stability. Shorter loops favor parallel multimers, while longer loops promote antiparallel, intramolecular forms, with potassium ions promoting more parallel structures.

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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • G-rich DNA sequences can form G-quadruplex structures.
  • Understanding G-quadruplex formation is crucial for various biological processes and therapeutic applications.

Purpose of the Study:

  • To investigate the impact of loop length on G-quadruplex structure and stability.
  • To explore the influence of different cations (K+, Na+, NH4+) on G-quadruplex conformation.

Main Methods:

  • Oligodeoxynucleotides with varying loop lengths (1-4 bases) and random bases (T or A) were synthesized.
  • Techniques included circular dichroism, native gel electrophoresis, UV-monitored thermal denaturation, and electrospray mass spectrometry.
  • Experiments were conducted in the presence of 150 mM K+, Na+, or NH4+.

Main Results:

  • Short loops (1-4 bases) favored parallel G-quadruplex conformations and the formation of stable dimers and trimers, even at low concentrations.
  • Increased loop length promoted intramolecular and antiparallel G-quadruplex conformations.
  • Cation type influenced structure, with K+ inducing more parallel multimers compared to NH4+ and Na+.

Conclusions:

  • Loop length is a critical determinant of G-quadruplex topology, affecting both intramolecular/intermolecular and parallel/antiparallel folding.
  • Cation selection provides another layer of control over G-quadruplex structure and assembly.
  • The study provides insights into higher-order G-quadruplex assemblies and their structural diversity.