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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Flow Cytometric Characterization of Murine B Cell Development
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Variability in molluscan hemocytes: a flow cytometric study.

K A Ashton-Alcox1, S E Ford

  • 1Rutgers University, Institute of Marine and Coastal Sciences New Jersey Agricultural Experiment Station, USA. kathryn@hsri.rutgers.edu

Tissue & Cell
|July 17, 2008
PubMed
Summary
This summary is machine-generated.

Flow cytometry reliably identifies oyster hemocyte subpopulations, including granular and agranular types. Individual metabolic condition, not environmental factors, likely drives hemocyte variability in marine bivalves.

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Area of Science:

  • Marine biology
  • Immunology
  • Cell biology

Background:

  • Marine bivalve hemocyte counts vary, potentially due to environmental factors and lab methods.
  • Flow cytometry offers automated analysis but requires confirmation of identified cell types.
  • Oyster hemocyte subpopulations identified by flow cytometry lack definitive cellular identification.

Purpose of the Study:

  • Confirm the identity of oyster hemocyte subpopulations identified by flow cytometry.
  • Investigate environmental factors (temperature, food) and individual variation influencing hemocyte proportions.
  • Assess the reliability of flow cytometry for oyster hemocyte analysis.

Main Methods:

  • Utilized light-scatter flow cytometry to differentiate oyster hemocyte subpopulations.
  • Employed cell sorting and microscopy to confirm the identity of sorted hemocytes.
  • Conducted a 4-week laboratory experiment manipulating temperature and food availability.

Main Results:

  • Flow cytometry consistently identified three oyster hemocyte subpopulations: granular, small granular, and agranular.
  • Microscopy confirmed these sorted cells as distinct hemocyte types.
  • Experimental manipulation of temperature and food did not significantly alter hemocyte counts or viability.
  • Significant correlation found between microscopy and flow cytometry estimates of subpopulation proportions.
  • Individual differences accounted for most hemocyte variability, mirroring vertebrate patterns.

Conclusions:

  • Flow cytometry is a validated tool for identifying and quantifying oyster hemocyte subpopulations.
  • Hemocyte variability in oysters is primarily linked to individual metabolic status rather than ambient environmental changes.
  • Further research should explore the physiological basis of individual metabolic condition's impact on hemocyte populations.