Related Experiment Video

Updated: Jul 3, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Potency of IMP-10 metallo-beta-lactamase in hydrolysing various antipseudomonal beta-lactams

Wei-Hua Zhao1, Zhi-Qing Hu1, Tadakatsu Shimamura1

  • 1Department of Microbiology and Immunology, Showa University School of Medicine, Tokyo 142-8555, Japan.

Journal of Medical Microbiology
|July 17, 2008
PubMed

Insights

Metallo-beta-lactamases (MBLs) like IMP-10 reduce the effectiveness of many antipseudomonal beta-lactams. This study reveals which antibiotics remain potent against Pseudomonas aeruginosa with IMP-10, aiding clinical selection.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • The emergence of metallo-beta-lactamases (MBLs), particularly IMP-type, poses a significant threat to treating Pseudomonas aeruginosa infections.
  • MBLs exhibit broad substrate specificity and resistance to available inhibitors, limiting the utility of beta-lactam antibiotics.

Purpose of the Study:

  • To assess the hydrolytic activity of IMP-10 MBL against clinically relevant antipseudomonal beta-lactams.
  • To determine the susceptibility profile of various beta-lactam antibiotics to IMP-10 hydrolysis.

Main Methods:

  • Crude IMP-10 MBL was isolated from clinical Pseudomonas aeruginosa strains carrying the bla(IMP-10) gene.
  • Minimum Inhibitory Concentrations (MICs) of 14 antipseudomonal beta-lactams were compared against a susceptible P. aeruginosa strain, with and without the presence of IMP-10 MBL.

Main Results:

  • Carbapenems (imipenem, meropenem, panipenem) and extended-spectrum cephems (ceftazidime, cefoperazone, cefsulodin, cefepime) were found to be sensitive to IMP-10 MBL hydrolysis.
  • Fourth-generation cephems (cefpirome), extended-spectrum penicillins (carbenicillin, ticarcillin, piperacillin, mezlocillin), and monobactams (aztreonam, carumonam) demonstrated relative resistance to IMP-10 MBL.

Conclusions:

  • The study provides a crucial sensitivity profile of antipseudomonal beta-lactams against IMP-10 MBL.
  • This information serves as a valuable reference for healthcare professionals in selecting appropriate antibiotics for Pseudomonas aeruginosa infections in the presence of IMP-10.
  • Understanding these resistance mechanisms is vital for effective antimicrobial stewardship.

More Related Videos

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases07:47

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...