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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jul 3, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Interactome data and databases: different types of protein interaction.

Javier De Las Rivas1, Alberto de Luis

  • 1Cancer Research Center (CIC, USAL-CSIC), University of Salamanca and CSIC, Campus Miguel de Unamuno, Salamanca E37007, Spain. jrivas@usal.es

Comparative and Functional Genomics
|July 17, 2008
PubMed
Summary
This summary is machine-generated.

High-throughput methods generate vast biological data, especially for protein-protein interactions (PPIs) and the interactome. This review covers recent PPI data, defines interaction types, and introduces the APIN tool for interactome browsing.

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Last Updated: Jul 3, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

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Area of Science:

  • Biomolecular Sciences
  • Bioinformatics
  • Computational Biology

Background:

  • High-throughput experimental and computational methods are advancing biomolecular sciences.
  • Massive datasets necessitate careful analysis for scientific knowledge extraction.
  • Protein-protein interaction (PPI) networks, or interactomes, are a key research area.

Purpose of the Study:

  • To review recent data and databases in protein-protein interaction research.
  • To provide a framework for understanding and classifying PPIs.
  • To introduce a new bioinformatic tool for interactome data exploration.

Main Methods:

  • Literature review of protein-protein interaction data and databases from the past five years.
  • Development of a conceptual scheme for defining and categorizing PPIs.
  • Presentation of the APIN (Agile Protein Interaction Network browser) tool.

Main Results:

  • Summary of key interactome data and databases.
  • A structured classification of protein-protein interaction types.
  • Introduction of APIN as a developing tool for interactome browsing.

Conclusions:

  • Accurate analysis of large-scale biological data is crucial.
  • Standardized definitions enhance the understanding of protein-protein interactions.
  • New bioinformatic tools like APIN are needed to navigate complex interactome data.