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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 3, 2026

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Published on: November 1, 2013

Early gene expression in wounded human keratinocytes revealed by DNA microarray analysis.

Manal A Dayem¹, Chimène Moreilhon, Laurent Turchi

¹Laboratoire de Physiologie Génomique des Eucaryotes, CNRS/UNSA UMR 6097, IPMC F-06560 Sophia Antipolis, France.

Comparative and Functional Genomics

|July 17, 2008

Summary

This study profiled gene expression during early wound healing in human keratinocytes using a novel wounding machine and DNA microarrays. Key genes involved in cell migration and proliferation were identified, validating the methodology for large-scale molecular event detection.

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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

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Last Updated: Jul 3, 2026

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Published on: November 1, 2013

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

Area of Science:

Molecular Biology
Cell Biology
Dermatology

Background:

Wound healing is a complex biological process involving cell spreading, migration, and proliferation.
Understanding the molecular mechanisms of early wound healing is crucial for developing effective treatments.

Purpose of the Study:

To validate gene expression profiling during early wound healing using a novel wounding machine and DNA microarrays.
To identify key molecular events and genes involved in the initial stages of wound repair in human keratinocytes.

Main Methods:

Utilized a custom-made DNA microarray with approximately 1000 human probes.
Employed an original wounding machine to create larger wounds (up to 40% surface area) in cultured human keratinocytes.
Analyzed gene expression profiles at multiple time points (0.5, 1.5, 3, 6, and 15 hours) post-wounding.

Main Results:

Gene expression patterns for c-Fos, c-Jun, Egr1, PLAU (uPA), and STAT3 were consistent with existing literature, validating the methodology.
Identified overexpression of transcripts for zinc finger proteins (ZFP36, ZNF161) and TNFAIP3 after wounding.
Investigated the role of p38 mitogen-activated protein kinase (p38MAPK) in wound healing, suggesting the existence of surrogate activating pathways.

Conclusions:

The developed methodology combining a novel wounding machine and DNA microarray analysis is effective for large-scale detection of molecular events in early wound healing.
The study identified specific genes and pathways, including p38MAPK, involved in the early cellular responses to wounding.
Findings provide a foundation for further research into the molecular regulation of wound repair.