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Updated: Jul 3, 2026

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
Published on: November 29, 2014
Synthetic peptide and protein domain arrays prepared by the SPOT technology
1Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt Universität zu Berlin, Berlin 10098, Germany. Schneider-mergener@jerini.de
Abstract:
The SPOT(trade mark) technology for highly parallel synthesis of peptides on flat surfaces in array type format has evolved into a versatile toolbox for a variety of applications in proteomics such as mapping protein-protein interactions and profiling the substrate specificity of enzymes such as kinases and proteases. Originally developed for the synthesis of short overlapping peptide sequences for mapping antibody epitopes this technology has recently been extended to the synthesis of functional protein domains. This opens up a variety of future applications such as target identification and protein expression profiling.
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