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Updated: Jul 3, 2026

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Published on: July 26, 2019
Haemophilus influenzae microarrays: virulence and vaccines
Tahir R Ali1, J Simon Kroll, Paul R Langford
1Department of Paediatrics, Faculty of Medicine, Imperial College of Science, Technology and Medicine, St Mary's Campus, London W2 1PG, UK.
Abstract:
In 1995 the genome sequence of the Haemophilus influenzae KW20 (Rd) strain was published, the first available for a free-living organism. The genome has been invaluable in global strategies to identify certain virulence-related genes, e.g. those involved in LPS synthesis, and also essential genes, but there is a paucity of wholegenome transcriptome studies. We have now constructed a whole-genome array consisting of genes from Rd, additional genes identified in other strains of H. influenzae and controls (from eukaryotic sources and other bacteria). We intend to use this array in studies aimed at understanding the bacterium's basic metabolism and its response to changing environments; deciphering global regulatory networks (by comparison of wild-type and mutant strains); and identifying genes expressed in vivo. The use of H. influenzae DNA arrays combined with proteomic approaches will enhance our understanding of the metabolism and virulence of the organism. Additionally, the genome sequence of a non-typable H. influenzae strain is in progress. The sequence from this isolate will be invaluable not only in identifying potential novel antibiotic targets and putative vaccine candidates but also in the design of a microarray for genome-typing purposes.
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