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Related Experiment Video

Updated: Jul 3, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

Survey sequencing and radiation hybrid mapping to construct comparative maps.

Christophe Hitte¹, Ewen F Kirkness, Elaine A Ostrander

¹CNRS, Université de Rennesl, Rennes Cédex, France.

Methods in Molecular Biology (Clifton, N.J.)

|July 17, 2008

Summary

Radiation hybrid (RH) mapping efficiently navigates genomes by breaking and capturing DNA. This technique creates ordered chromosome maps, aiding comparative genomics across species.

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Published on: June 28, 2012

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Last Updated: Jul 3, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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Published on: August 12, 2019

Comparative Lesions Analysis Through a Targeted Sequencing Approach

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Published on: November 5, 2019

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Radiation hybrid (RH) mapping is a key technique for genome navigation.
It involves random chromosome breakage and capture by recipient cells.

Purpose of the Study:

To outline the principles and applications of RH mapping.
To highlight its efficiency in generating ordered genome maps.

Main Methods:

Inducing random chromosome breakage in a target genome.
Capturing DNA fragments in recipient cells.
Analyzing markers via PCR and statistical tools.

Main Results:

RH mapping establishes linear order and confidence of marker placement.
High-resolution maps comparable to deep sequencing can be generated.
Combined with FISH, RH maps provide complete chromosomal blueprints.

Conclusions:

RH mapping is an economical and efficient method for genome navigation.
It facilitates comparative genomics by ordering genes and markers.
The technique is versatile and applicable beyond mammalian genomes.