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Cell harvesting by cross-flow microfiltration using a shear-enhanced module.

U Frenander1, A S Jönsson

  • 1Chemical Engineering 1, Lund University, S-221 00 Lund, Sweden.

Biotechnology and Bioengineering
|November 5, 1996
PubMed
Summary
This summary is machine-generated.

A novel rotational shear filter efficiently separated Vibrio cholerae protein from fermentation broth. This method achieved high protein recovery (97%) and flux, demonstrating its effectiveness for bioprocessing.

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Separation Science

Background:

  • Recombinant protein production in bacteria like Vibrio cholerae is crucial for various applications.
  • Efficient separation of target proteins from fermentation broth is a key challenge in bioprocessing.
  • Conventional microfiltration methods can face limitations in protein recovery and flux.

Purpose of the Study:

  • To evaluate a new mechanically agitated (rotational) shear filter for separating recombinant Vibrio cholerae protein.
  • To assess the filter's performance in terms of protein recovery, permeate flux, and transmembrane pressure.
  • To determine the filter's efficiency during concentration and diafiltration steps.

Main Methods:

  • Utilized a DMF(TM) rotational shear filter for cross-flow microfiltration of bacterial fermentation broth.
  • Employed a process of sixfold concentration followed by twofold diafiltration.
  • Monitored transmembrane pressure, permeate flux, and protein transmission throughout the separation process.
  • Evaluated filter cleaning efficacy using an enzymatic cleaning agent.

Main Results:

  • Achieved higher protein recovery (97%) and permeate flux compared to common cell harvesting methods.
  • Maintained a constant flux of 150 L/m(2) h with only a 10 kPa transmembrane pressure increase.
  • Demonstrated high protein transmission: ~100% initially, >90% post-concentration, and >80% during diafiltration.
  • Observed no significant decrease in pure water flux after enzymatic cleaning, indicating good filter cleanability.

Conclusions:

  • The DMF(TM) rotational shear filter offers superior performance for Vibrio cholerae protein separation.
  • The method enables efficient concentration and diafiltration with high protein recovery and stable operation.
  • This technology presents a promising advancement for bioseparation processes in the pharmaceutical and biotechnology industries.