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[Effect of c-ski on biological behavior of rat skin-derived fibroblast]
1Molecular Biology Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Objective:
To observe the effect of oncogene c-ski on the cell viability, myofibroblast differentiation and type I collagen secretion of rat skin-derived fibroblast.
Methods:
In situ expression of c-Ski in fibroblast was detected by immunohistochemistry. After cells transfected with c-ski gene, the changes of cell proliferation was measured with the method of MTT, the type I collagen secretion and myofibroblast differentiation were checked with the methods of immunohistochemistry and RT-PCR.
Results:
There was obvious expression of c-Ski in fibroblast, which mainly located in nucleus. c-ski could promote cell proliferation in a dose-dependent manner, and also decrease mRNA and protein expression of type I collagen, but had no effect on myofibrblast differentiation.
Conclusion:
The roles of c-ski promoting cell viability and decreasing collagen secretion may have an important significance in accelerating wound healing and reducing scar formation.
