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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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Related Experiment Video

Updated: Jul 3, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

U12-dependent intron splicing in plants.

C G Simpson1, J W S Brown

  • 1Scottish Crop Research Institute, Invergowrie, Dundee Scotland, UK. g.g.simpson@dundee.ac.uk

Current Topics in Microbiology and Immunology
|July 18, 2008
PubMed
Summary
This summary is machine-generated.

U12-dependent (U12) introns are rare, essential gene regulators in plants, utilizing a unique spliceosome. Plant U12 introns share similarities with other eukaryotes but possess plant-specific features, suggesting unique regulatory roles.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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Last Updated: Jul 3, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • U12-dependent (U12) introns are ancient genetic elements conserved since early eukaryotic evolution.
  • These introns are rare but crucial for regulating essential genes involved in DNA replication and RNA metabolism.
  • U12 introns are processed by a distinct spliceosome, differing significantly from the U2-dependent spliceosome.

Purpose of the Study:

  • To compare U12 and U2 intron splicing mechanisms in plants.
  • To review the characteristics of plant U12 introns and their potential role in gene expression regulation.
  • To investigate the evolutionary persistence and unique features of U12 introns in plant genomes.

Main Methods:

  • Comparative analysis of U12 and U2 intron splicing pathways.
  • Review of existing literature on plant U12 introns and their splicing machinery.
  • Examination of conserved and unique features in plant U12 intron sequences.

Main Results:

  • Plant U12 introns exhibit conserved splicing signals similar to other eukaryotes.
  • Plant U12 introns possess unique characteristics, such as UA-richness, distinct from typical U2 introns.
  • The U12 intron-specific spliceosome shares components with the U2 spliceosome but requires unique snRNAs.

Conclusions:

  • Plant U12 introns are highly conserved but also possess plant-specific attributes.
  • These unique features suggest the involvement of plant-specific factors in both U12 and U2 splicing.
  • U12 introns play a significant role in regulating gene expression in plants, warranting further investigation.