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Related Concept Videos

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model01:09

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model

Various dissolution theories provide insight into the factors that influence the dissolution rate. Danckwerts' Model suggests that turbulence, rather than a stagnant layer, characterizes the dissolution medium at the solid-liquid interface. In this model, the agitated solvent contains macroscopic packets that move to the interface via eddy currents, facilitating the absorption and delivery of the drug to the bulk solution. The regular replenishment of solvent packets maintains the concentration...
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Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
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Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...
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Updated: Jul 3, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Protein diffusion across the interface in aqueous two-phase systems.

Götz Münchow1, Friedhelm Schönfeld, Steffen Hardt

  • 1Institut fur Mikrotechnik Mainz GmbH, Carl-Zeiss-Strasse 18-20, Mainz, Germany. muenchow@imm-mainz.de

Langmuir : the ACS Journal of Surfaces and Colloids
|July 18, 2008
PubMed
Summary

Protein transport across fluid phase boundaries is not significantly affected by local boundary conditions. Our study on aqueous two-phase systems found no retardation of diffusive transport, indicating minimal interfacial influence.

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Area of Science:

  • Biophysics
  • Physical Chemistry
  • Chemical Engineering

Background:

  • Diffusive transport of proteins is crucial in biological and chemical processes.
  • Aqueous two-phase systems (ATPS) are widely used for protein separation.
  • Interfacial phenomena at phase boundaries can potentially alter mass transfer.

Purpose of the Study:

  • To investigate if local effects at the fluid phase boundary retard protein diffusion in ATPS.
  • To determine the significance of protein adsorption and electric double layers on interfacial mass transfer.
  • To compare experimental findings with a theoretical model.

Main Methods:

  • Utilized a microfluidic system for precise control and observation.
  • Analyzed diffusion of bovine serum albumin and ovalbumin in polyethylene glycol/dextran ATPS.
  • Developed a one-dimensional model including phase-specific diffusion and chemical potential differences.

Main Results:

  • Experimental data showed good agreement with simulation results.
  • Observed no significant retardation of protein diffusion across the phase boundary.
  • Concluded that local phase boundary influences on protein transport are negligible in this system.

Conclusions:

  • The phase boundary in these aqueous two-phase systems does not impede protein diffusion.
  • Protein adsorption and electric fields at the interface do not significantly alter transport rates.
  • Findings suggest robust diffusive transport of proteins in ATPS under studied conditions.