Related Experiment Video
Updated: Jul 3, 2026

Preparation of Free-Surface Hyperbolic Water Vortices
Published on: July 28, 2023
The shock of the old: hydrodynamics for the masses
1National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, UK. dj.scott@nottingham.ac.uk
Abstract:
Hydrodynamic techniques such as analytical ultracentrifugation can provide key information about subunit stoichiometry and interaction strengths of protein-nucleic acid interactions. Analysis is complicated by (i) the need for low concentrations in order to observe both free and bound species and (ii) thermodynamic non-ideality. With the introduction of fluorescence optics, we are able to obtain data at lower concentrations, and improved understanding of the statistical thermodynamics of macromolecular solutions has allowed non-ideality to be accurately assigned. With these developments, it is possible now to assay protein-nucleic acid interactions at concentrations typically used in molecular biology assays.
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