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Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
Crystal Growth: Principles of Crystallization01:25

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Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

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Published on: March 10, 2023

Higher-throughput approaches to crystallization and crystal structure determination.

Mark J Fogg1, Anthony J Wilkinson

  • 1Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, UK.

Biochemical Society Transactions
|July 18, 2008
PubMed
Summary

Structural genomics initiatives have accelerated protein structure determination, leading to advances in high-throughput techniques from gene to protein structure. These methods enhance the speed and efficiency of structural biology research.

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Area of Science:

  • Structural biology
  • Genomics
  • Biochemistry

Background:

  • The Protein Data Bank has seen a significant increase in deposited protein structures.
  • Structural genomics initiatives are a major driver of this expansion.
  • Advances in high-throughput structural determination techniques are crucial.

Purpose of the Study:

  • To discuss recent advances in the pipeline of protein structure determination.
  • To highlight the impact of structural genomics on high-throughput methods.
  • To showcase activities at the York Structural Biology Laboratory within a European consortium.

Main Methods:

  • Ligation-independent cloning methods
  • Parallel purification systems
  • Robotic crystallization devices
  • Automated methods for crystal identification, data collection, and structure solution

Main Results:

  • Significant progress in high-throughput protein structure determination techniques.
  • Successful implementation of automated and robotic systems in structural biology workflows.
  • Contribution to the growing public repository of protein structures.

Conclusions:

  • Technological advancements are rapidly increasing the pace of protein structure determination.
  • Structural genomics plays a vital role in driving innovation in structural biology.
  • Collaborative efforts, like the Structural Proteomics in Europe consortium, are essential for progress.