Related Experiment Video
Updated: Jul 3, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
3'-Terminal nucleotide sequence (N = 41) of satellite tobacco necrosis virus RNA
J Merregaert1, J V Emmelo, W Fiers
1Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium.
Abstract:
To convert satellite tobacco necrosis virus RNA into a template for complementary DNA (cDNA) synthesis, a poly(A) tail was added to its 3'-end by means of Escherichia coli ATP:RNA adenyltransferase. This product could then be copied by avian myeloblastosis virus RNA-dependent DNA polymerase in the presence of p(dT)(10) as primer. Under appropriate conditions, cDNA was synthesized discontinuously, and limited transcripts were obtained when one or more deoxynucleoside triphosphates were omitted from the reaction mixture. "Phased" cDNA fragments suitable for sequence analysis were synthesized when (dT)(8)dG, (32)P-labeled at the 5'-end, was used as a primer. We report here the sequence of the 3'-terminal 41 nucleotides of the viral RNA.
More Related Videos
13:07Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
12:20Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Related Concept Videos
Subviral Agents
Antiviral Nucleoside Inhibitors
Viruses with RNA Genomes
Nucleic Acid Structure
DNA Structure
DNA has a double-helix structure. The...
RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...