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Related Experiment Video

Updated: Jul 3, 2026

Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

Published on: April 9, 2018

Transfection with baculovirus DNA.

J P Burand¹, M D Summers, G E Smith

¹Department of Entomology, Texas A&M University, College Station, Texas 77843, USA.

|February 1, 1980

Summary

Infectious DNA from Autographa californica (AcMNPV) and Rachiplusia ou (RoMNPV) nuclear polyhedrosis viruses can infect TN-368 cells. AcMNPV DNA demonstrated higher infectivity than RoMNPV DNA in these baculovirus transfection experiments.

Area of Science:

Molecular Virology
Insect Pathology
Baculovirus Research

Background:

Nuclear polyhedrosis viruses (NPVs) are important insect pathogens.
Understanding baculovirus DNA infectivity is crucial for gene expression studies and pest control applications.

Purpose of the Study:

To investigate the infectivity of purified Autographa californica NPV (AcMNPV) and Rachiplusia ou NPV (RoMNPV) DNA in TN-368 cells.
To compare the transfection efficiency of different baculovirus DNAs and DNA forms.

Main Methods:

Purified AcMNPV and RoMNPV DNA were used for transfection in TN-368 cells.
The calcium phosphate precipitation technique was employed for DNA delivery.
Plaque-forming units (PFU) were quantified to determine DNA infectivity.

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Last Updated: Jul 3, 2026

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Main Results:

Both AcMNPV and RoMNPV DNAs were infectious in TN-368 cells, unlike other tested baculovirus DNAs.
AcMNPV DNA transfection yielded 3600 PFU/µg DNA, while RoMNPV DNA yielded 2900 PFU/µg DNA.
AcMNPV Form I DNA was four times more infectious than Form II DNA in TN-368 cells.

Conclusions:

TN-368 cells are susceptible to transfection by AcMNPV and RoMNPV DNA using the calcium phosphate method.
AcMNPV DNA exhibits higher infectivity than RoMNPV DNA in this cell line.
DNA conformation (Form I vs. Form II) significantly impacts AcMNPV DNA infectivity.