Related Experiment Video
Updated: Jul 3, 2026
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Experimental antimicrobial orthodontic adhesives using nanofillers and silver nanoparticles
Sug-Joon Ahn1, Shin-Jae Lee, Joong-Ki Kook
1Dental Research Institute and Department of Orthodontics, College of Dentistry, Seoul National University, 28-22 Yunkeun-Dong, Chongro-Ku, Seoul 110-768, Republic of Korea.
Objectives:
Experimental composite adhesives (ECAs) containing silica nanofillers and silver nanoparticles were compared with two conventional adhesives (composite and resin-modified glass ionomer [RMGI]) to analyze surface characteristics, physical properties and antibacterial activities against cariogenic streptococci.
Methods:
Surface roughness and surface free energy (SFE) characteristics were measured using confocal laser scanning microscopy and the sessile drop method. Shear bond strength and bond failure interface were analyzed to compare the physical properties. Antimicrobial activities were analyzed by a bacterial adhesion assay, a disk diffusion test, and an optical density measurement of bacterial suspension containing each adhesive.
Results:
ECAs had rougher surfaces than conventional adhesives due to the addition of silver nanoparticles. ECAs had more similar SFE characteristics to composite than to RMGI. Bacterial adhesion to ECAs was less than to conventional adhesives, which was not influenced by saliva coating. Bacterial suspension containing ECAs showed slower bacterial growth than those containing conventional adhesives. There was no significant difference in shear bond strength and bond failure interface between ECAs and conventional adhesives.
Significance:
This study suggests that ECAs can help prevent enamel demineralization around their surfaces without compromising physical properties.
