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Modulation of bladder myofibroblast activity: implications for bladder function.

Gui-Ping Sui1, Changhao Wu, Alexander Roosen

  • 1Postgraduate Medical School, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.

American Journal of Physiology. Renal Physiology
|July 18, 2008
PubMed
Summary
This summary is machine-generated.

Suburothelial myofibroblasts in the bladder wall respond to stimuli and intercellular contact. Glivec selectively reduces spontaneous bladder activity in spinal cord-transected rats, suggesting a therapeutic target for overactivity.

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Area of Science:

  • Urology
  • Cell Biology
  • Physiology

Background:

  • Bladder suburothelial myofibroblasts influence bladder sensory input and activity.
  • Understanding their response to external agents is crucial for bladder function.

Purpose of the Study:

  • To characterize suburothelial myofibroblast responses to agents affecting bladder activity.
  • To investigate the role of intercellular contact and potential drug targets.

Main Methods:

  • Experiments on guinea pig and rat bladder tissues (detrusor strips, sheets) and isolated myofibroblasts.
  • Measurements included isometric tension, intracellular calcium (Ca2+), and membrane currents.
  • Cell pair formation and drug treatments (ATP, UTP, pH, capsaicin, sodium nitroprusside, Glivec) were employed.

Main Results:

  • Suburothelial myofibroblasts responded to ATP, UTP, and reduced pH with Ca2+ transients and inward currents.
  • Intercellular contact augmented these responses and lowered activation thresholds.
  • Glivec inhibited these augmentations and reduced spontaneous activity in spinal cord-transected rat bladders, affecting Ca2+ wave spread.

Conclusions:

  • Suburothelial myofibroblasts are responsive to various stimuli, with their activity modulated by physical contact.
  • Glivec's selective action on spontaneous activity in spinal cord-transected rats highlights a potential therapeutic pathway for bladder overactivity.