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Collection and Identification of Pollen from Honey Bee Colonies
Published on: January 19, 2021
A unique caleosin in oil bodies of lily pollen
Pei-Luen Jiang1, Guang-Yuh Jauh, Co-Shing Wang
1Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.
Abstract:
In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.
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