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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Enumeration of antigen sites on cells by flow cytometry

C D Dawson1, J A Scheppler, J K Nicholson

  • 1Immunology Branch, Centers for Disease Control, Atlanta, Ga.

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
|January 1, 1991
PubMed

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...