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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jul 3, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

High-throughput quantitative real-time PCR.

Zoltan P Arany¹

¹Cardiovascular Institute, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Human Genetics

|July 18, 2008

Summary

This summary is machine-generated.

Miniaturization of quantitative real-time PCR (qRT-PCR) enables high-throughput analysis of nucleic acids. This study presents a simple method to quantify 1880 selected transcripts from RNA samples.

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Published on: August 3, 2011

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Quantitative real-time PCR (qRT-PCR) has undergone significant technical advancements.
Miniaturization has made qRT-PCR suitable for high-throughput applications.
The technique allows for rapid and quantitative measurement of numerous nucleic acids.

Purpose of the Study:

To describe a simple technique for quantifying a large number of transcripts.
To enable high-throughput analysis of gene expression and other nucleic acids.
To quantify 1880 specific transcripts from any number of RNA samples.

Main Methods:

Development of a miniaturized quantitative real-time PCR (qRT-PCR) assay.
Application of the technique to quantify 1880 selected transcripts.
Adaptability for analyzing gene expression across multiple samples or multiple genes in few samples.

Main Results:

Successful quantification of 1880 transcripts of choice.
Demonstration of the technique's applicability to various investigative needs.
High-throughput capacity for nucleic acid quantification.

Conclusions:

The described technique offers a simple and efficient method for high-throughput transcript quantification.
This approach benefits diverse research areas, including gene expression analysis.
Miniaturized qRT-PCR significantly enhances the scale and speed of molecular analyses.