Related Experiment Video
Updated: Jul 3, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
An integrated metabolic model for the aerobic and denitrifying biological phosphorus removal
E Murnleitner1, T Kuba, M C van Loosdrecht
1Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Abstract:
In this work, an integrated metabolic model for biological phosphorus removal is presented. Using a previously proposed mathematical model it was shown to be possible to describe the two known biological phosphorus removal processes, under aerobic and denitrifying conditions, with the same biochemical reactions, where only the difference in electron acceptor (oxygen and nitrate) is taken into account. Though, apart from the ATP/NADH ratio, the stoichiometry in those models is identical, different kinetic parameters were found. Therefore, a new kinetic structure is proposed that adequately describes phosphorus removal under denitrifying and aerobic conditions with the same kinetic equations and parameters. The ATP/NADH ratio (delta) is the only model parameter that is different for aerobic and denitrifying growth. With the new model, simulations of anaerobic/aerobic and anaerobic/denitrifying sequencing batch reactors (A(2) SBR and A/O SBR) were made for verification of the model. Not only short-term behavior, but also steady state, was simulated. The results showed very good agreement between model predictions and experimental results for a wide range of dynamic conditions and sludge retention times. Sensitivity analysis shows the influence of the model parameters and the feed substrate concentrations on both systems.
Related Concept Videos
Microbial Wastewater Treatment
Metabolism of Chemolithotrophs
Biological Treatment of Effluent and Waste Water
The Phosphorus Cycle
Microbes and the Nitrogen Cycle
Microbial Bioremediation of Uranium

