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Related Experiment Video

Updated: Jul 3, 2026

Microinjection of Xenopus Laevis Oocytes

Microinjection of Xenopus Laevis Oocytes

Published on: February 23, 2009

Okra mosaic virus empty protein shells in nuclei.

B Marshall¹, R E Matthews

¹Department of Cell Biology, University of Auckland, Auckland, New Zealand.

|April 15, 1981

Summary

Okra mosaic virus causes empty viral protein shells to accumulate in cucumber nuclei. This suggests viral coat protein enters the nucleus and assembles into shells within the nuclear environment.

Area of Science:

Plant virology
Molecular biology
Cellular biology

Background:

Okra mosaic virus (OMV) is a plant pathogen that infects various crops.
Viral protein shells play a crucial role in virus structure and replication.
Understanding viral assembly processes is key to developing disease control strategies.

Purpose of the Study:

To investigate the localization and assembly of Okra mosaic virus (OMV) protein shells in infected cucumber cotyledons.
To determine the specific viral components that accumulate in the nucleus.
To elucidate the mechanism of viral protein shell formation within the host cell.

Main Methods:

Inoculation of cucumber cotyledons with Okra mosaic virus.
Analysis of viral protein shell accumulation using microscopy and biochemical assays.

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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Fractionation of cellular components to isolate and examine nuclear contents.

Main Results:

Large quantities of empty viral protein shells (approx. 10 mg/g tissue) were produced by OMV in cucumber.
Empty protein shells, but not intact virus particles, were detected accumulating within isolated nuclei.
Approximately half of the total viral protein shells were found in the nucleus one day post-inoculation, even with virus particles present in the cytoplasm.

Conclusions:

Okra mosaic virus (OMV) preferentially accumulates empty protein shells in the nucleus.
Viral coat protein likely enters the nucleus as monomers or small oligomers (pentamers, hexamers).
Assembly of viral protein shells into complete structures occurs preferentially within the nuclear compartment.