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Updated: Jul 3, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Structure of the 19 S RNA transcript encoded by the cauliflower mosaic virus genome
J T Odell1, R Keith Dudley, S H Howell
1Biology Department C-016, University of California, San Diego, La Jolla, California 92093, USA.
Abstract:
Turnip leaves infected with cauliflower mosaic virus accumulate several virus-derived RNA species. These include a large transcript (>25 S) and a 19 S transcript. R-looping and diazo-cellulose filter hybridization techniques show that the 19 S RNA is derived from a contiguous segment of the CaMV genome which includes EcoR1-b and -d DNA fragments. This region encodes the synthesis of a nonvirion protein called P66. The 3' end of the 19 S transcript is polyadenylated and terminates near the single strand break in the alpha-strand of the genome. The 5' end of the transcript starts in or near the EcoR1-d fragment, near position zero on the map described by Hull and Howell (1978).
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