Related Experiment Video
Updated: Jul 3, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
Published on: May 11, 2020
Low-molecular-weight RNAs associated with tobacco ringspot virus are satellites
1Department of Biology, Shiraz University, Shiraz, Iran.
Abstract:
The low-molecular-weight RNA associated with an isolate of tobacco ringspot virus [TRSV(F)] lacks polyadenylic acid [poly(A)] sequences. Complementary DNA (cDNA) specific to this RNA was made with an oligo(dT) primer after tailing the RNA with poly(A). However, a significant amount of cDNA was made only when the RNA was treated with alkaline phosphatase prior to tailing. Hybridization of RNA in solution and by a Northern blot technique showed that there was no detectable nucleotide sequence homology between TRSV(F) RNA and two of the low-molecular-weight RNAs associated with this virus. These associated RNAs are therefore, satellites of TRSV(F). The relationship of these satellites to a previously reported satellite of TRSV is discussed.
Related Concept Videos
Subviral Agents
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

