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Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
Published on: August 11, 2023
Gene expression profiling of oral submucous fibrosis using oligonucleotide microarray
Yanjia Hu1, Xinchun Jian, Jieying Peng
1Department of Oral and Maxillofacial Surgery, Xiang Ya Hospital, Central South University, 410008 Changsha, People's Republic of China.
Oncology Reports
|July 19, 2008
Summary
Oral submucous fibrosis (OSF), a precancerous lesion linked to betel quid chewing, involves distinct gene expression changes. Immune, inflammatory, and TGF-beta-induced epithelial-mesenchymal transition pathways are implicated in OSF development and malignancy.
Area of Science:
- Genomics
- Molecular Biology
- Oncology
Background:
- Oral submucous fibrosis (OSF) is a prevalent precancerous oral lesion in Southeast Asia, strongly associated with betel quid (BQ) chewing.
- The molecular mechanisms underlying OSF pathogenesis and malignant transformation remain poorly understood.
Purpose of the Study:
- To identify genes involved in the pathogenesis and malignant transformation of oral submucous fibrosis (OSF).
- To analyze gene expression profiles in OSF using oligonucleotide microarrays.
Main Methods:
- Oligonucleotide microarray analysis (Affymetrix U133A 2.0 GeneChip) of 14,500 genes in human OSF and normal oral tissues.
- Gene Ontology (GO) analysis and bioinformatics tools to identify differentially expressed genes.
- RT-PCR validation of selected epithelial-mesenchymal transition (EMT)-related genes.
Main Results:
- Significant differences in gene expression profiles were observed between OSF and normal tissues.
- 716 genes were upregulated and 149 genes were downregulated in OSF.
- Differentially expressed genes were primarily associated with immune response, inflammatory response, and TGF-beta-induced EMT.
Conclusions:
- Aberrant gene expression in immune response, inflammatory response, and TGF-beta-induced EMT pathways likely contributes to OSF pathogenesis.
- These molecular alterations may play a crucial role in the malignant transformation of OSF.
- Specific EMT-related genes (SFRP4, THBS1, MMP2, ZO-1, CK18) were identified and validated.
