Related Experiment Video

Updated: Jul 3, 2026

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

mda-7/IL-24 induces apoptosis in human HepG2 hepatoma cells by endoplasmic reticulum stress

Xiaofeng Zhang1, Xiaoyan Kang, Lehua Shi

  • 1Department of Molecular Oncology, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai 200438, People's Republic of China.

Oncology Reports
|July 19, 2008
PubMed

Insights

Adenovirus-mediated mda-7/IL-24 (Ad.mda-7) induces apoptosis in liver cancer cells by activating endoplasmic reticulum (ER) stress. Blocking ER stress inhibits Ad.mda-7

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • The tumor suppressor gene mda-7/IL-24 exhibits broad-spectrum anticancer activity.
  • The precise molecular mechanisms underlying mda-7/IL-24-induced apoptosis remain incompletely understood and may vary by tumor type.

Purpose of the Study:

  • To investigate the apoptotic effects of adenovirus-mediated mda-7/IL-24 (Ad.mda-7) on human HepG2 hepatoma cells.
  • To elucidate the role of endoplasmic reticulum (ER) stress in Ad.mda-7-induced apoptosis in liver cancer.

Main Methods:

  • Adenovirus-mediated delivery of mda-7/IL-24 (Ad.mda-7) to HepG2 cells in vitro.
  • Inhibition of ER stress pathways to assess its impact on apoptosis.
  • In vivo studies using subcutaneous HepG2 tumor xenografts in mice.
  • Analysis of apoptosis-related gene expression (caspase-12, Bax, caspase-3).

Main Results:

  • Ad.mda-7 treatment induced apoptosis in HepG2 cells.
  • Blocking ER stress significantly inhibited Ad.mda-7-induced apoptosis and down-regulated key apoptotic markers (caspase-12, Bax, caspase-3).
  • Ad.mda-7 treatment inhibited tumor growth and angiogenesis in vivo, with ER stress inhibition preventing apoptosis in liver cancer cells.

Conclusions:

  • Ad.mda-7 primarily induces apoptosis in HepG2 liver cancer cells through the activation of the endoplasmic reticulum stress pathway.
  • Targeting ER stress represents a potential therapeutic strategy in conjunction with Ad.mda-7 for liver cancer treatment.

More Related Videos

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

Related Concept Videos

Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...