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Related Concept Videos

Filtration00:53

Filtration

Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...
Laminar Flow01:27

Laminar Flow

Laminar flow represents a smooth, orderly fluid motion where particles move along parallel paths, resulting in minimal mixing between layers. Streamlined particle paths characterize this flow regime and occur under conditions where viscous forces dominate over inertial forces. The distinction between laminar, transitional, and turbulent flow is primarily determined by the Reynolds number, a dimensionless quantity calculated as:

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Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
12:47

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

High performance tangential flow filtration.

R van Reis1, S Gadam, L N Frautschy

  • 1Separation Technology Group, Department of Recovery Sciences, Genentech, Inc, 460 Point San Bruno Boulevard, South San Francisco, California 94080, USA. rvr@gene.com

Biotechnology and Bioengineering
|July 19, 2008
PubMed
Summary
This summary is machine-generated.

High Performance Tangential Flow Filtration (HPTFF) overcomes limitations in traditional methods, enabling precise protein-protein separations. This advanced filtration technology offers competitive purification for the biotechnology industry.

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Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Separation Science

Background:

  • Conventional tangential flow filtration (TFF) is limited to separating molecules with large size differences (approx. tenfold).
  • Challenges include wide pore-size distributions, membrane fouling, and concentration polarization, restricting TFF applications in biotechnology.
  • Current TFF use is mainly for cell-protein, virus-protein, and protein-buffer separations.

Purpose of the Study:

  • To overcome limitations of conventional TFF for achieving high-resolution protein-protein separations.
  • To develop and implement High Performance TFF (HPTFF) systems for advanced purification.
  • To enhance TFF's utility in the biotechnology industry.

Main Methods:

  • Developed new membrane formulations and casting techniques to improve pore-size distribution.
  • Controlled membrane fouling by operating in the transmembrane pressure-dependent filtrate flux regime and optimizing start-up conditions.
  • Exploited and controlled concentration polarization using a co-current filtrate stream for constant transmembrane pressure.

Main Results:

  • Achieved high yields and purification factors, even for proteins with minimal sieving differences.
  • Successfully separated immunoglobulin G (IgG) from bovine serum albumin (BSA) mixtures.
  • Demonstrated separation of BSA monomer-oligomer mixtures using HPTFF.

Conclusions:

  • HPTFF technology significantly enhances separation capabilities beyond conventional TFF.
  • The developed methods effectively address membrane fouling and concentration polarization.
  • HPTFF presents a competitive purification alternative to complement existing chromatographic methods in protein processing.