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Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

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Protocols for Robust Herbicide Resistance Testing in Different Weed Species
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Transcriptome response to glyphosate in sensitive and resistant soybean.

Jin Zhu1, William L Patzoldt, Robin T Shealy

  • 1Department of Crop Sciences, University of Illinois, Urbana, Illinois 61801, USA. tranel@uiuc.edu

Journal of Agricultural and Food Chemistry
|July 19, 2008
PubMed
Summary

Glyphosate-resistant soybeans show minimal gene expression changes after glyphosate exposure. This suggests current genetically modified soybeans are not significantly impacted by this herbicide at the transcriptomic level.

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Genetics

Background:

  • Most US soybeans are glyphosate-resistant due to a gene for a glyphosate-insensitive 5-enolpyruvylshikimate-3-phosphate synthase.
  • Concerns exist regarding potential secondary effects of glyphosate on soybean physiology.

Purpose of the Study:

  • To investigate the transcriptomic effects of glyphosate on both glyphosate-sensitive and glyphosate-resistant soybean lines.
  • To determine if glyphosate induces significant gene expression changes in resistant soybean varieties.

Main Methods:

  • Gene expression profiling using cDNA microarrays.
  • Treatment of soybean plants with glyphosate at different time points (1, 4, and 24 hours post-treatment).
  • Quantitative real-time reverse-transcribed Polymerase Chain Reaction (qRT-PCR) for validation.

Main Results:

  • Glyphosate-sensitive plants showed significant differential gene expression (3, 170, 311 genes at 1, 4, 24 hpt).
  • Glyphosate-resistant plants exhibited minimal changes (10, 4, 4 genes at 1, 4, 24 hpt), indicating rapid adjustment.
  • Comparison of developing seeds revealed negligible differences between resistant and sensitive lines.

Conclusions:

  • Glyphosate-resistant soybeans demonstrate resilience to glyphosate treatment at the transcriptomic level.
  • No major transcriptomic alterations were observed in current glyphosate-resistant soybean varieties.
  • The study supports the safety of current glyphosate-resistant soybean technology regarding gene expression impacts.