Related Experiment Video
Updated: Jul 3, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor
Leon Caly1, Nitin K Saksena, Sabine C Piller
1Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia. leon.caly@med.monash.edu.au
Abstract:
We previously reported an epidemiologically linked HIV-1 infected patient cohort in which a long-term non-progressor (LTNP) infected two recipients who then exhibited normal disease progression. Expression of patient-derived vpr sequences from each of the three cohort members in mammalian cells tagged with GFP revealed a significant reduction in Vpr nuclear import and virion incorporation uniquely from the LTNP, whereas Vpr from the two progressing recipients displayed normal localisation and virion incorporation, implying a link between efficient Vpr nuclear import and HIV disease progression. Importantly, an F72L point mutation in the LTNP was identified for the first time as being uniquely responsible for decreased Vpr nuclear import.
Related Concept Videos
Inhibitors of Virion Maturation and Assembly
Size and Structure of Viral Genomes
Retrovirus Life Cycles
Retroviruses
Viral Mutations
Inhibitors Of Virion Release

