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Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands
Published on: October 2, 2018
Leukemia inhibitory factor protein and receptors are expressed in the bovine adrenal cortex and increase cortisol and
Alison M Woods1, Christopher J McIlmoil, Ernestina N Rankin
1Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT 84602, USA.
Domestic Animal Endocrinology
|July 22, 2008
Summary
Leukemia inhibitory factor (LIF) regulates adrenal steroid release in cows. LIF stimulates cortisol release and inhibits androgen release, suggesting a role in stress response.
Area of Science:
- Endocrinology
- Molecular Biology
- Neuroscience
Background:
- Adrenal steroid release is regulated by ACTH during acute stress.
- Chronic inflammatory stress involves additional factors, including Leukemia inhibitory factor (LIF).
- LIF and its receptors (LIFR) are present in human adrenal cortex and H295R cells, influencing cortisol release.
Purpose of the Study:
- To investigate LIF and LIFR expression in the bovine adrenal cortex.
- To determine the effects of LIF on cortisol and androgen release from bovine adrenal cells.
- To elucidate LIF's role in regulating adrenal steroidogenesis during inflammatory stress.
Main Methods:
- Immunohistochemistry
- Western blots
- RT-PCR
- Nucleotide sequencing
- Cell dispersion and incubation assays
Main Results:
- LIF and LIFR are expressed throughout the bovine adrenal cortex.
- LIF enhanced ACTH-stimulated cortisol release from zona fasciculata cells.
- LIF inhibited basal and ACTH-stimulated androgen release from zona reticularis cells.
Conclusions:
- LIF is present in the bovine adrenal cortex and influences steroid release.
- LIF stimulates cortisol release and inhibits androgen release, suggesting a role in inflammatory stress response.
- LIF may be a key regulator of adrenal steroidogenesis during chronic inflammatory conditions.
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