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Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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Cellular Membranes and Drug Transport

Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
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Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport01:23

Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport

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Vesicular Tubular Clusters01:45

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Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
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Introduction to Membrane Traffic

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Super-resolution Imaging of Neuronal Dense-core Vesicles
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Dendritic multishell architectures for drug and dye transport.

Mohiuddin A Quadir1, Michał R Radowski, Felix Kratz

  • 1Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|July 22, 2008
PubMed
Summary

Newly developed core-multishell nanoparticles (CMS NPs) efficiently encapsulate and transport antitumor drugs like doxorubicin hydrochloride and methotrexate, along with dyes for in vivo imaging. These versatile nanoparticles show stable interactions and promising tumor contrast in preclinical studies.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Core-multishell nanoparticles (CMS NPs) offer advanced drug delivery potential.
  • Developing versatile nanocarriers for simultaneous drug and dye encapsulation is crucial for theranostics.

Purpose of the Study:

  • To evaluate the efficiency and versatility of novel CMS NPs for encapsulating and transporting antitumor drugs and dye molecules.
  • To characterize the interaction of CMS NPs with various payloads using multiple analytical techniques.
  • To assess the in vivo imaging capabilities of dye-loaded CMS NPs in a tumor-bearing mouse model.

Main Methods:

  • Size exclusion chromatography (SEC) and UV-VIS spectroscopy for encapsulation and transport evaluation.
  • Isothermal titration calorimetry (ITC) to study drug-nanoparticle interactions.
  • Nuclear magnetic resonance (NMR) spectroscopy for payload-nanoparticle interaction analysis.
  • In vivo imaging studies in F9 teratocarcinoma bearing mice.

Main Results:

  • CMS NPs demonstrated spontaneous encapsulation and transport of doxorubicin hydrochloride, methotrexate, and nile red in organic and aqueous media.
  • Isothermal titration calorimetry revealed a stable, exothermic interaction between sodium ibandronate and CMS NPs (ΔH ≈ 7 kcals/mol).
  • NMR experiments confirmed the interaction of sodium ibandronate with CMS NPs.
  • CMS NPs encapsulated tetrasulfonated indotricarbocyanine dye with a stoichiometry of 6-8 molecules per nanocarrier.
  • In vivo imaging showed strong contrast in tumor tissues after 6 hours with dye-loaded CMS NPs compared to free dye.

Conclusions:

  • The developed CMS NPs are efficient and versatile nanocarriers for both antitumor drugs and imaging dyes.
  • CMS NPs exhibit stable encapsulation and transport capabilities, confirmed by various spectroscopic and calorimetric techniques.
  • Dye-loaded CMS NPs show potential for effective in vivo tumor imaging, highlighting their theranostic applications.