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Physicochemical properties of potato virus M

S M Tavantzis1

  • 1Department of Botany and Plant Pathology, University of Maine, Orono, Maine 04469, USA.

Virology
|March 1, 1984
PubMed

Insights

Potato virus M (PVM), a carlavirus, has a single capsid protein and a single-stranded RNA genome. PVM infection is associated with virus-specific double-stranded RNA, aiding in its characterization.

Area of Science:

  • Virology
  • Plant Pathology
  • Molecular Biology

Background:

  • Potato virus M (PVM) is a significant pathogen within the carlavirus group.
  • Understanding PVM's physical and molecular properties is crucial for disease management and control strategies.

Purpose of the Study:

  • To characterize the physical and molecular properties of Potato virus M (PVM).
  • To elucidate the genomic and structural composition of PVM.

Main Methods:

  • Sedimentation analysis to determine sedimentation coefficient (S20,w).
  • Buoyant density measurements in Cesium Chloride (CsCl).
  • Spectrophotometric analysis for extinction coefficient and nucleic acid content determination.
  • Molecular weight determination of the genome and capsid polypeptide.

Main Results:

  • PVM exhibited a sedimentation coefficient (S20,w) of 157 S and a buoyant density of 1.322 g.cm(-3).
  • The nucleic acid content was 6.0%, with a single-stranded RNA genome (MW 2.5 x 10^6) containing polyadenylate.
  • A single capsid polypeptide (MW 35.7 x 10^3) and virus-specific double-stranded RNA were identified.

Conclusions:

  • PVM is characterized by specific biophysical parameters and a defined molecular composition.
  • The presence of double-stranded RNA is a consistent marker for PVM infection.
  • These findings contribute to the fundamental understanding of PVM and carlaviruses.

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