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Lymphocyte activation by cell separation procedures.

R C Wardley, B T Rouse, L A Babiuk

    Immunological Communications
    |January 1, 1976
    PubMed
    Summary
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    Standard cell separation methods can unintentionally activate lymphocytes, making them cytotoxic. This activation occurs when cells pass through specific columns with fresh plasma or serum, impacting immunological studies.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lymphocyte separation is crucial for immunological research.
    • Standard techniques may inadvertently alter cell function.

    Purpose of the Study:

    • To investigate the cytotoxic effects induced by standard cell separation procedures.
    • To understand the conditions and mechanisms underlying this induced cytotoxicity.

    Main Methods:

    • Lymphocytes were passed through glass wool and/or nylon wool columns.
    • Columns were equilibrated with media containing fresh plasma or serum.
    • Cell activation and cytotoxicity were assessed.

    Main Results:

    • Cell separation under specific conditions induced lymphocyte cytotoxicity against target cells, including autologous lymphocytes.

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  • Cytotoxicity required passage through wool columns with fresh plasma/serum.
  • Activated cells lost cytotoxic potential after overnight culture or trypsin treatment.
  • Separation also released heat-labile cytotoxic factors and antibodies.

    • Conclusions:

    • Standard cell separation techniques can induce unintended lymphocyte cytotoxicity.
    • This phenomenon necessitates careful interpretation of cell separation studies.
    • The release of cytotoxic factors and antibodies also impacts study outcomes.