Development of acetylcholinesterase biosensor based on CdTe quantum dots/gold nanoparticles modified chitosan...

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biosensor Technology

Background:

• Acetylcholinesterase (AChE) is a crucial enzyme in nerve function.
• Pesticide detection is vital for food safety and environmental monitoring.
• Developing sensitive and rapid detection methods for pesticides like monocrotophos is essential.

Purpose of the Study:

• To construct a novel electrochemical biosensor for monocrotophos detection.
• To utilize CdTe quantum dots (QDs) and gold nanoparticles (GNPs) for enhanced sensitivity.
• To immobilize AChE on a modified glassy carbon electrode for a stable biosensing platform.

Main Methods:

• Modification of a glassy carbon electrode with CdTe QDs and GNPs via chitosan microspheres.
• Immobilization of acetylcholinesterase (AChE) onto the modified electrode surface.
• Electrochemical detection of monocrotophos based on AChE inhibition.

Main Results:

• The developed CdTe QDs-GNPs composite biosensor demonstrated synergistic effects, enhancing detection sensitivity.
• The biosensor achieved a low detection limit of 0.3 ng mL(-1) for monocrotophos.
• The method showed good precision, reproducibility, stability, and accuracy in analyzing real samples (garlic).

Conclusions:

• The novel AChE biosensor based on CdTe QDs-GNPs composite is a sensitive, rapid, and cost-effective tool for monocrotophos determination.
• The synergistic interaction between CdTe QDs and GNPs significantly amplifies the detection signal.
• This biosensing platform holds promise for practical applications in pesticide residue analysis.