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Updated: Jul 3, 2026
08:56
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Evidence for replicative recombination in cauliflower mosaic virus
L Dixon1, T Nyffenegger, G Delley
1Friedrich Miescher-Institut, P.O. Box 2543, CH-1,002 Basel, Switzerland.
Virology
|April 30, 1986
Summary
DNA sequencing of Cauliflower mosaic virus (CaMV) strain CM4-184 shows it contains genetic material from CaMV strains S and CM1841. Recombination likely occurred during reverse transcription via intergenomic switching between viral RNA templates.
Area of Science:
- Plant virology
- Molecular biology
- Genetics
Background:
- Cauliflower mosaic virus (CaMV) is a significant plant pathogen.
- CaMV exhibits genetic variability and recombination.
Purpose of the Study:
- To analyze the genetic makeup of CaMV strain CM4-184.
- To investigate the origins and recombination mechanisms of CaMV strain CM4-184.
Main Methods:
- DNA sequencing of CaMV strain CM4-184.
- Comparative analysis of viral genomic regions.
Main Results:
- CaMV strain CM4-184 possesses distinct genetic regions homologous to CaMV strains S and CM1841.
- The identified recombination sites suggest intergenomic switching during reverse transcription of the minus-strand DNA.
Conclusions:
- CaMV strain CM4-184 is a recombinant strain.
- The findings elucidate a potential mechanism for CaMV strain evolution and diversification.
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