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The primary structure of red clover mottle virus middle component RNA
M Shanks1, J Stanley, G P Lomonossoff
1John Innes Institute, Colney Lane, Norwich NR/, 7UH, United Kingdom.
Abstract:
The primary structure of middle component RNA of red clover mottle virus (RCMV) has been determined. The RNA is polyadenylated, has a small protein (VPg) covalently linked to its 5' terminus and consists of 3543 nucleotides. The RNA possesses a single long open reading frame which can code for a polypeptide of molecular weight 109,452. Comparison of the 3' terminal sequence of the M RNA with that of the homologous B RNA reveals a high degree of sequence conservation in this area. Computer-assisted comparisons show that the polypeptides encoded by the M RNAs of RCMV and CPMV (cowpea mosaic virus) are highly homologous implying that the two viruses have a common genetic structure.
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