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Updated: Jul 3, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Genetic differences among Staphylococcus aureus isolates from dairy ruminant species: a single-dye DNA microarray

E Vautor¹, V Magnone, G Rios

¹Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité Pathologie des Petits Ruminants, Sophia-Antipolis, France. e.vautor@sophia.afssa.fr

Veterinary Microbiology

|July 22, 2008

Summary

Staphylococcus aureus virulence genes show host-specific traits in small ruminants and cattle. Genomic analysis reveals distinct gene profiles linked to lineage and host, impacting pathogen development.

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Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach

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Last Updated: Jul 3, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

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Published on: November 4, 2016

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach

Published on: October 7, 2025

Area of Science:

Veterinary Microbiology
Genomics
Bacterial Pathogenesis

Background:

Staphylococcus aureus is a significant pathogen causing intramammary infections in livestock.
Understanding virulence factors is crucial for controlling infections in sheep, goats, and cows.

Purpose of the Study:

To compare virulence gene profiles of S. aureus isolates from dairy sheep, goats, and cows.
To investigate potential lineage- and host-specific genetic traits of S. aureus.

Main Methods:

DNA microarray analysis to detect 190 virulence factors.
Polymerase Chain Reaction (PCR) for targeted gene assessment.
Pulse-field gel electrophoresis (PFGE) and spa typing for isolate characterization.

Main Results:

A dominant clone (OV/OV', spa-CC 1773) found in ovine and caprine isolates, associated with agr group III and specific virulence genes (lpl4, ssl6, bsaA1, bsaB, bsaP, SAV0812).
Small ruminant isolates showed unique associations with genes like lpl1, sel, sec, tst, lukM, and SAV0876.
Bovine isolates were associated with the egc cluster, fhuD1, abiF, and SAV2496.

Conclusions:

Genomic data suggests the presence of lineage- and host-specific virulence genes in S. aureus.
These genetic differences may contribute to the development of host-specific pathogenic traits.