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Updated: Jul 3, 2026
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Genetic differences among Staphylococcus aureus isolates from dairy ruminant species: a single-dye DNA microarray
1Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité Pathologie des Petits Ruminants, Sophia-Antipolis, France. e.vautor@sophia.afssa.fr
Veterinary Microbiology
|July 22, 2008
Summary
Staphylococcus aureus virulence genes show host-specific traits in small ruminants and cattle. Genomic analysis reveals distinct gene profiles linked to lineage and host, impacting pathogen development.
Area of Science:
- Veterinary Microbiology
- Genomics
- Bacterial Pathogenesis
Background:
- Staphylococcus aureus is a significant pathogen causing intramammary infections in livestock.
- Understanding virulence factors is crucial for controlling infections in sheep, goats, and cows.
Purpose of the Study:
- To compare virulence gene profiles of S. aureus isolates from dairy sheep, goats, and cows.
- To investigate potential lineage- and host-specific genetic traits of S. aureus.
Main Methods:
- DNA microarray analysis to detect 190 virulence factors.
- Polymerase Chain Reaction (PCR) for targeted gene assessment.
- Pulse-field gel electrophoresis (PFGE) and spa typing for isolate characterization.
Main Results:
- A dominant clone (OV/OV', spa-CC 1773) found in ovine and caprine isolates, associated with agr group III and specific virulence genes (lpl4, ssl6, bsaA1, bsaB, bsaP, SAV0812).
- Small ruminant isolates showed unique associations with genes like lpl1, sel, sec, tst, lukM, and SAV0876.
- Bovine isolates were associated with the egc cluster, fhuD1, abiF, and SAV2496.
Conclusions:
- Genomic data suggests the presence of lineage- and host-specific virulence genes in S. aureus.
- These genetic differences may contribute to the development of host-specific pathogenic traits.
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