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Updated: Jul 3, 2026

Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughs
Tomohiro Kaino1, Tetsuya Tateiwa, Satomi Mizukami-Murata
1Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Abstract:
We constructed self-cloning diploid baker's yeast strains by disrupting PUT1, encoding proline oxidase, and replacing the wild-type PRO1, encoding gamma-glutamyl kinase, with a pro1(D154N) or pro1(I150T) allele. The resultant strains accumulated intracellular proline and retained higher-level fermentation abilities in the frozen doughs than the wild-type strain. These results suggest that proline-accumulating baker's yeast is suitable for frozen-dough baking.

