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Dynamic Light-Induced Protein Patterns at Model Membranes
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Dynamic Light-Induced Protein Patterns at Model Membranes

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Protein dynamics under light control

Michele Vendruscolo1

  • 1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. mv245@cam.ac.uk

Nature Chemical Biology
|July 22, 2008
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...