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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jul 3, 2026

A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Immunolabeling of embryos.

H-Arno J Müller¹

¹Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Scotland UK.

Methods in Molecular Biology (Clifton, N.J.)

|July 22, 2008

Summary

This study presents a versatile immunolabeling protocol for Drosophila embryos, optimizing antibody penetration and structural preservation for accurate subcellular protein localization in developmental biology research.

Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

Drosophila embryogenesis is a well-established model for studying animal development.
Research focus is shifting from developmental patterning to the cell biology of morphogenesis.
Determining subcellular protein localization via immunolabeling is crucial for understanding these processes.

Purpose of the Study:

To provide a general immunolabeling protocol for Drosophila embryos.
To offer variations for optimizing antibody penetration and structural preservation.
To facilitate the study of subcellular protein localization for a wide range of antigens.

Main Methods:

Development of whole mount-staining procedures for Drosophila embryos.
Balancing structural preservation during fixation with antibody penetration.

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A Method for Labeling Vasculature in Embryonic Mice

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Utilizing specific fixation and staining variations to preserve cellular compartments.

Main Results:

A general immunolabeling protocol is established.
Variations are presented to accommodate diverse antigens and optimize results.
The protocol aims to improve the accuracy of subcellular protein localization studies.

Conclusions:

Effective immunolabeling protocols are essential for advancing the cell biological understanding of Drosophila morphogenesis.
The presented protocol offers a flexible approach for researchers studying protein localization in embryos.
This method supports detailed investigations into the molecular mechanisms of development.