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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jul 3, 2026

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Immunolabeling of imaginal discs.

Thomas Klein1

  • 1Institute for Genetics, University of Cologne, Cologne, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|July 22, 2008
PubMed
Summary

This protocol details a rapid method for immunolabeling Drosophila imaginal discs. Researchers can efficiently analyze protein expression and localization in a single day, aiding developmental biology studies.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Drosophila melanogaster imaginal discs are crucial models for studying signaling pathways and cellular development.
  • Immunolabeling is essential for visualizing protein expression and localization in these tissues.

Purpose of the Study:

  • To present a streamlined protocol for dissecting, fixing, and immunolabeling Drosophila imaginal discs.
  • To enable researchers to perform the entire procedure, including image acquisition, within one day.

Main Methods:

  • Dissection of Drosophila imaginal discs.
  • Fixation of dissected imaginal discs.
  • Immunolabeling using fluorochrome-conjugated antibodies.
  • Image acquisition for analysis.

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Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae
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Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Published on: February 17, 2007

Related Experiment Videos

Last Updated: Jul 3, 2026

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae
07:36

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Published on: February 17, 2007

Main Results:

  • A complete protocol for immunolabeling imaginal discs is provided.
  • The protocol is optimized for a one-day procedure.
  • Successful visualization of protein expression patterns and subcellular localization is demonstrated.

Conclusions:

  • This rapid immunolabeling protocol enhances the efficiency of studying Drosophila imaginal discs.
  • It serves as a valuable tool for researchers investigating developmental and cell biological processes.