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Related Experiment Video

Updated: Jul 3, 2026

Imaging Through the Pupal Case of Drosophila melanogaster
06:50

Imaging Through the Pupal Case of Drosophila melanogaster

Published on: January 23, 2014

Imaging Drosophila pupal wing morphogenesis.

Anne-Kathrin Classen1, Benoit Aigouy, Angela Giangrande

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 22, 2008
PubMed
Summary

We present optimized protocols for preparing Drosophila pupal wings for microscopy. These methods enable detailed visualization of epithelial architecture and live imaging of glial cell dynamics during development.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Microscopy Techniques

Background:

  • Drosophila pupal wing development involves complex epithelial and glial morphogenesis.
  • Understanding these dynamic events is crucial for cell biologists.
  • Existing protocols may limit detailed visualization or live imaging.

Purpose of the Study:

  • To establish optimized protocols for preparing Drosophila pupal and prepupal wings for immunofluorescence microscopy.
  • To enable detailed visualization of wing epithelial architecture, including planar cell polarity.
  • To provide a method for whole-mount live imaging of pupal wings, focusing on glial cell behavior.

Main Methods:

  • Protocol development for immunofluorescence microscopy of Drosophila pupal (P) and prepupal (PP) wings.

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A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
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A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Published on: December 30, 2017

Live-imaging of the Drosophila Pupal Eye
09:54

Live-imaging of the Drosophila Pupal Eye

Published on: January 12, 2015

Related Experiment Videos

Last Updated: Jul 3, 2026

Imaging Through the Pupal Case of Drosophila melanogaster
06:50

Imaging Through the Pupal Case of Drosophila melanogaster

Published on: January 23, 2014

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
07:11

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Published on: December 30, 2017

Live-imaging of the Drosophila Pupal Eye
09:54

Live-imaging of the Drosophila Pupal Eye

Published on: January 12, 2015

  • Optimization of sample preparation for visualizing epithelial structures.
  • Development of a whole-mount live imaging protocol for P wings.

    • Main Results:

    • Successfully visualized polarized cortical domains of planar cell polarity proteins in wing epithelial architecture.
    • Enabled live imaging of glial cell migration and proliferation along wing sensory nerves.
    • Demonstrated the utility of the developed protocols for studying dynamic developmental processes.

    Conclusions:

    • The presented protocols significantly advance the study of Drosophila wing development.
    • These methods facilitate detailed analysis of epithelial organization and cell dynamics.
    • The protocols are valuable tools for cell biologists investigating morphogenesis and cell migration.