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Updated: Jul 3, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
[ALL 98 strains of Brucella OMP25 gene is conservative in China]
Wen-jing Wang1, Ming Li, Yong-yu Rui
1School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
Objective:
To investigate the genetic polymorphism of OMP25 gene isolated from 116 Brucella strains, including 18 Brucella reference strains and 98 Chinese field strains.
Methods:
Chromosomal DNA of Brucella strains were analyzed by PCR, and then the product OMP25 gene was digested with Hind III and separated on 10% polypropylene agarose gel electrophoresis. OMP25 genes of different types were sequenced.
Results:
Eight gene types (I-VIII) existing in 116 strains were examined, among them type II and VII were only found in certain wild strains. Sequencing analysis of OMP25 revealed only 10 sites varied in all 8 types. OMP25 of 98 Chinese Brucella strains was highly homologic and characteristic of stable antigen.
Conclusion:
High conservation characteristics helps to epidemiologically detect Brucella in China as well as to found a theoretical basis for vaccine development.
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